1111 Topical γ-linolenic acid restores skin barrier dysfunction and ameliorates atopic dermatitis in hairless mice

Atopic dermatitis (AD) is a common pruritic inflammatory skin disease. Skin barrier dysfunction plays pivotal role in AD development. A decrease acylceramides the stratum corneum may contribute to AD. However, few highly effective therapies for exist. We have reported unique, diet-induced mouse model; Hairless mice fed special diet (HR-AD) showed and, consequently, AD-like dermatitis. The was caused mainly by deficiency of polyunsaturated fatty acids (PUFA). Abnormal PUFA metabolism has been implicated human Many studies investigated efficacy oral supplementation PUFA, such as evening primrose oil and borage rich γ-linolenic acid (GLA), on manifestations; however, results conflicting. these oils contain only 10-20% GLA, examined effects topical purified GLA symptoms. Therefore, present study aimed investigate symptoms our model its mechanism. After developing symptoms, 2.5% GLA-containing ointment applied whole surface (200 mg/mouse) once daily seven days. Topical application restored with increasing covalently bound ceramides corneum. Comprehensive gene expression analysis revealed that regulated epidermal differentiation complex genes, Sprr2d S100a8. Furthermore, suppressed increased thymic stromal lymphopoietin, interleukin (IL)-4, IL-5, IL-13, CCL17. Consequently, ameliorated inflammation itch-related scratching behavior. In conclusion, show can restore suppress type 2 immune responses thus be therapeutic candidate